Author Guidelines

Language Quality Requirement

Please note that your manuscript will not be processed unless it is of sufficient quality of the English language. If you feel that your paper could benefit from English language polishing, we strongly recommend that you consider having your paper professionally edited for English language by a native English speaker or a service such as Wiley Editing Services. Please note that while this service will greatly improve the readability of your paper, it does not guarantee acceptance of your paper by the journal.

All manuscripts must be submitted in English and should be as concise as possible. Either standard American spelling and grammar or standard British spelling and grammar are acceptable, but each manuscript must conform to only one of the two styles. Merriam Webster’s Collegiate Dictionary (10th ed.) should be followed for matters of standard American (English) spelling, compound words, and word division. The Concise Oxford Dictionary of Current English (Clarendon Press, Oxford) should be followed for questions of British (English) spelling and grammar.

Editorial policy

Biotechnology and Applied Biochemistry publishes original full-length articles, short communications, and reviews relating to biological and process-engineering sciences that underpin the translation of discoveries in life sciences to therapeutic and diagnostic products for the benefit of mankind. Reviews provide the reader with timely updates and perspectives in fast-moving areas of medical biotechnology.

The focus of the journal is directed towards the publication of original articles and reviews concerning the topics: applied aspects of cellular physiology and metabolism of bacteria, fungi, animal, and plant cells; animal-cell biotechnology; plant-cell biotechnology; biochemical engineering; bioseparation and other downstream processes including chromatography; systems biology and synthetic biology; gene and protein therapy; biomaterials; regenerative medicine, stem cells and tissue engineering; analytical biotechnology including bio-imaging and bio-sensing; nano-biotechnology; bioactivity and toxicity mechanism studies including apoptosis. Original articles must contain sufficient detail to enable others to repeat the work.

Manuscripts are accepted for review with the understanding that the same work has not been published elsewhere in any language, including publication on the World Wide Web (WWW); that it is not under consideration for publication elsewhere; that its submission for publication has been approved by all of the authors and by the institution where the work was carried out; that all persons entitled to authorship have been so named; and that any person cited as a source of personal communication has approved such citation. Written authorization may be required at the Editor's direction. Abstracts of oral or poster presentations are not considered to constitute previous publication. Preliminary communications in journals that regularly publish reports in this form will not preclude publication of a paper in Biotechnology and Applied Biochemistry, provided the full paper contains additional information that justifies its publication and does not repeat the presentation of the same data. To facilitate evaluation of this matter by the Editors, submitted manuscripts should be accompanied by copies of all preliminary communications and of all relevant manuscripts that are in press or under editorial consideration elsewhere.

Submission of a paper implies that authors will make available samples of unique biological materials (including cell lines, DNA clones and antibodies) to academic workers who request them.

The description of experimental procedures must be rigorous. For example, the term 'room temperature' is to be avoided; actual temperature or temperature ranges must be reported. It is expected that authors will have validated the use of published experimental procedures for the specific use stated in the submitted manuscript. The description of experimental procedures must be of sufficient quality to comply with the principles of Good Laboratory Practice or the geographical equivalent.

Authors are encouraged to suggest a suitable member of the Editorial Board and up to five potential reviewers of international standing for their paper. Authors may also specify the names of those they wish to be excluded from the review process for a particular paper; in such cases their wishes will usually be respected, unless, of course, in the opinion of the journal such a request unreasonably excludes all the expertise available to it in that scientific area.

Biotechnology and Applied Biochemistry follows a rigorous reviewing procedure, which, for each paper, generally requires written input from two independent referees. The Editorial Office, if required, receives advice before selecting the team of people involved in each paper and in each case the Editorial Office ensures that those involved in the process act independently and have no conflict of interest in the paper. In case of a complaint, the Editorial Office, if it is deemed necessary and appropriate, may ask for additional review(s) before making a recommendation. In all cases, the final decision rests with the Editor-in-Chief. The Editorial Board reserves the right to reject papers that are unsuitable for the journal or cannot adequately be assessed because of a poor standard of English.


Biotechnology and Applied Biochemistry endorses the Vancouver Guidelines on authorship as defined in the International Committee of Medical Journal Editors' (ICMJE) Uniform Requirements for Manuscripts Submitted to Biomedical Journals, namely that entitlement to authorship should be based on all of the following criteria: (1) substantial contributions to conception and design, or acquisition of data, or analysis and interpretation of data; (2) drafting the article or revising it for important intellectual content;(3) final approval of the version to be published. Acquisition of funding, collection of data, or general supervision of the research group, alone, does not justify authorship. All contributors who do not meet the criteria for authorship should be listed in the Acknowledgements.


It is a condition of publication that authors assign copyright or license the publication rights in their articles, including abstracts to Biotechnology and Applied Biochemistry. This enables us to ensure full copyright protection and to disseminate the article, and of course the Journal, to the widest possible readership in print and electronic formats as appropriate. Authors may, of course, use the article elsewhere after publication without prior permission from John Wiley & Sons, Inc., provided that acknowledgement is given to the Journal as the original source of publication, and that John Wiley & Sons, Inc., is notified so that our records show that its use is properly authorized. Authors are themselves responsible for obtaining permission to reproduce copyright material from other sources.
Click here for the Copyright Transfer Agreement.

Note to NIH Grantees

Pursuant to NIH mandate, Wiley-Blackwell will post the accepted version of contributions authored by NIH grant-holders to PubMed Central upon acceptance. This accepted version will be made publicly available 12 months after publication. For further information, see

Supplemental Material

Biotechnology and Applied Biochemistry Online offers authors the opportunity to enhance their papers with supplemental material (e.g. time-lapse movies, three-dimensional structures) in online publication. These will be submitted to peer review alongside the manuscript. To submit a paper with a multimedia adjunct, attach the file when you submit your manuscript online. Preferred formats are QuickTime for time-lapse movies and PDB for structures. There is no extra charge associated with the publication of supplemental material online.

Submission of Manuscripts

The journal to which you are submitting your manuscript employs a plagiarism detection system. By submitting your manuscript to this journal you accept that your manuscript may be screened for plagiarism against previously published works.

All submissions should be made online at Biotechnology & Applied Biochemistry ScholarOne Manuscripts site. New users must first create an account. Once a user is logged onto the site submissions should be made via the Author Center. For assistance with any aspect of the site, please refer to the User Guide, which is accessed via the ’Get Help Now’ button at the top right of every screen.
All manuscripts must be written in English. The names, addresses, telephone, fax numbers and e-mail addresses of at least four knowledgeable, well-qualified, and independent reviewers must be provided.
Authors are responsible for obtaining permission to reproduce copyrighted material, including published data and extensive quotations, from other sources. Authors are also required to sign an agreement to transfer the copyright for their manuscript to the IUBMB. The Publisher will supply this form upon manuscript acceptance. All accepted manuscripts, artwork, and photographs become the property of the publisher.

Form of manuscript

Manuscripts should be written in clear, concise, and grammatically correct English; manuscripts that are inadequately prepared will be declined, since it is not feasible for the Editors to undertake extensive revision or rewriting of manuscripts. Editorial style should follow that recommended in the Instructions to Authors of the Biochemical Journal (see Nomenclature, abbreviations and symbols should wherever possible follow the recommendations of the Nomenclature Committee of IUBMB and the IUPAC-IUBMB Joint Commission on Biochemical Nomenclature (see The International System of Units (SI; see Quantities, Units and Symbols in Physical Chemistry, 3rd edn, 2007, RSC Publishing, Cambridge) should be used; however, certain non-SI units (e.g. angstrom, minute) will continue to be accepted.
Page 1 should contain the article title, name(s) of author(s) (with first or second given name spelled out in full), affiliation(s), a short running title (abbreviated form of title) of less than 50 characters including spaces, and the name and complete mailing address (including telephone and fax numbers and e-mail address) of the person to whom correspondence should be sent.
Page 2 should contain a short synopsis of 50-250 words, and any footnotes to the text. A list of up to six key words (in alphabetical order), of which at least three do not appear in the title of the paper, should form the first footnote, and a list of abbreviations used should form the second; neither of these two footnotes should bear a symbol. Other footnotes, identified by superscript arabic numerals, should be limited to dedications, an identification of the corresponding author and an author's present address.
The recommended organization of an article is: (i) synopsis, (ii) introduction, (iii) materials and methods (or experimental), (iv) results, (v) discussion, (vi) acknowledgements (vii) funding (in the form of a sentence with the funding agency written out in full followed by the grant number in square brackets) and (viii) references. Combination of certain of these sections (e.g. results and discussion) may make the presentation clearer.

Addresses for correspondence

All correspondence about papers should be sent to:

Editorial Office
Biotechnology and Applied Biochemistry
111 River Street, Hoboken, NJ 07030
Telephone: 210-748-7760

Experimental and publishing ethics

Experiments with animals should be performed in accordance with the legal requirements of the relevant local or national authority. Procedures should be such that experimental animals do not suffer unnecessarily. The text of papers should include details of the strain or stock of animal used, experimental procedures, and of anaesthetics used. It is recommended that authors refer to the 'ARRIVE' guidelineson the reporting of animal research.

Papers describing any experimental work with humans should include a statement that the research has been carried out in accordance with the Declaration of Helsinki (2000) of the World Medical Association, that the Ethical Committee of the Institution in which the work was performed has approved it, and that the subjects have given informed consent to the work.

Biotechnology and Applied Biochemistry is a member of COPE (Committee on Publication Ethics) and endorses its guidelines, which are available at The journal also endorses the guidelines published by the International Union of Biochemistry and Molecular Biology (IUBMB; Code of Ethics). Complaints against the Journal must be submitted in writing to the Editor-in-Chief; if a complaint is not resolved to the satisfaction of the complainant they have the option of referring the matter to COPE. Notwithstanding, the Editorial Board will not accept papers where the ethical aspects are, in the Board's opinion, open to doubt.

Biotechnology and Applied Biochemistry endorses the guidelines published by COPE (Committee on Publication Ethics; and will always investigate fully any matters of apparent misconduct that it becomes aware of.

Biotechnology and Applied Biochemistry follows the guidelines published by COPE with respect to the retraction of articles.

Biotechnology and Applied Biochemistry will not tolerate plagiarism in submitted manuscripts. Passages quoted or closely paraphrased from other authors (or from the submitting author's own published work) must be identified as quotations or paraphrases, and the sources of the quoted or paraphrased material must be acknowledged. Use of unacknowledged sources will be construed as plagiarism. If any manuscript is found to contain plagiarized material the review process will be halted immediately.


Tables should be located at the end of text, after the References section, and should also be uploaded as a .doc or .docx file seperate from the manuscript file. Tables should be prepared using the Microsoft Word table editor and should be numbered with arabic numerals (Table 1, Table 2, etc.) and cited consecutively in the text. Each table should be titled. Units must be clearly indicated for each of the entries in the table. Footnotes to tables should be identified by superscript lower-case roman letters and placed at the bottom of the table.

Table specifications:

In addition to being located in the manuscript file, each table must be uploaded as a separate file when submitting a manuscript, and must be in .doc or .docx form.


No artwork should be incorporated into the text files. Figures should be submitted as separate files and cited consecutively in the text by arabic numerals (Figure 1, Figure 2, etc.). The use of tints should be avoided. Lettering on the figures should be of a size that allows for eventual reduction of the figures. Authors will be required to pay a fee of $500 per page for figures to be printed in color.

Figure Specifications:

Each Figure must be uploaded as a separate file when submitting a manuscript, and must be in TIFF or EPS format. Figures are not to be included in the main manuscript file.

The minimum requirements for proper resolution are:

300 DPI/PPI for halftones (CMYK/Grayscale; figures that contain pictures only without text labeling or lines);

600 DPI/PPI for combination halftones (figures that contain pictures with text labeling and/or thin lines);

1200 DPI/PPI for monochrome (figures that are entirely black and white; line graphs are an example)

In-line equations should be typed as text. Displayed equations (unless prepared by the 'MathType Equation Editor') are re-keyed by our typesetter.

Image acquisition and manipulation

Images will be checked for manipulation when a paper is accepted. The Editorial Board may request that authors supply the original data for comparison against the prepared figures. If authors are unable to comply with such a request, the acceptance of the paper may be withdrawn.

Biotechnology and Applied Biochemistry endorses the guidelines given in the Instructions for Authors of the Journal of Cell Biology, from where the following is adapted by kind permission of Rockefeller University Press:

The following information must be provided about the acquisition and processing of images:

1. Make and model of microscope
2. Type, magnification, and numerical aperture of the objective lenses
3. Temperature
4. Imaging medium
5. Fluorochromes
6. Camera make and model
7. Acquisition software
8. Any subsequent software used for image processing, with details about types of operations involved (e.g. type of deconvolution, 3D reconstructions, surface or volume rendering, gamma adjustments, etc.).

No specific feature within an image may be enhanced, obscured, moved, removed or introduced. The grouping of images from different parts of the same gel, or from different gels, fields or exposures must be made explicit by the arrangement of the figure (i.e. using dividing lines) and in the text of the figure legend. Adjustments of brightness, contrast or color balance are acceptable if they are applied to the whole image and as long as they do not obscure, eliminate or misrepresent any information present in the original, including backgrounds. The background of figures should be clearly distinct from the surrounding page. Non-linear adjustments (e.g. changes to gamma settings) must be disclosed in the figure legend.

Authors are encouraged to read the papers by M. Rossner and K. M. Yamada (2004) J. Cell Biol. 166, 11-15and A. J. North (2006) J. Cell Biol. 172, 9-18.


References should be cited in numerical order in parentheses (eg. (1) or (1-3)). If a reference is cited more than once the same number should be used each time. The references should be listed at the end of the article in numerical order in the following form:

Journal article[1] Gal, S., Klein, J.W. (2000) Biochem. Mol. Biol. Educ. 28, 267-271.

Book[2] Strunk Jr., W., White, E.B. (1979) The Elements of Style, 3rd ed., Macmillan, New York.

Edited book[3] Letheridge, S., Cannon, C.R., Eds. (1980) Bilingual Education: Teaching English as a Second Language, Praeger, New York.

Article or chapter in edited book[4] Gurman, A.S., Kniskem, D.P., in Gurman, A.S., Kniskem, D.P., Eds. (1981) Handbook of Family Therapy, Brunner/Maazel, New York, pp. 742-775.

Proceedings[5] Chaddock, T.E., in: Daniel, E.E., Ed. (1974) Proceedings of the Fourth International Symposium on Gastrointestinal Motility, Mitchell Press, Vancouver, British Columbia, Canada, pp. 83-92.

Preprint[6] Yu, F., Wu, X.-S. (1992) Physicss Review Letters 68 2996(hep-th/911209).

URL[7] World Index of Molecular Visualization Resources: (revised 2001)


One set of proofs will be sent to the corresponding author in form of a PDF file. Changes made at this time should reflect corrections of typographical or factual errors. Proofs must be carefully checked and returned within 48 hours of receipt. Articles will be added to an issue in the order that the publisher receives the proofs. Failure to return proofs within 48 hours may result in the article being delayed for publication until a later issue.


Reprints may be purchased at

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