ORIGINAL ARTICLE
Evaluation of growth and some unexplored bioactivities of bioreactor grown adventitious root culture of ginseng (Panax ginseng C.A. Meyer)
Sium Ahmed,
Abdullah Mohammad Shohael,
Kee Yoeup Paek,
Sium Ahmed
Cell Genetics and Plant Biotechnology Laboratory, Department of Biotechnology and Genetic Engineering, Jahangirnagar University, Savar, Dhaka, 1342 Bangladesh
Search for more papers by this authorCorresponding Author
Abdullah Mohammad Shohael
Cell Genetics and Plant Biotechnology Laboratory, Department of Biotechnology and Genetic Engineering, Jahangirnagar University, Savar, Dhaka, 1342 Bangladesh
Correspondence
Abdullah Mohammad Shohael, Department of Biotechnology and Genetic Engineering, Jahangirnagar University, Savar, Dhaka-1342, Bangladesh.
Email: [email protected]
Search for more papers by this authorKee Yoeup Paek
Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University, Cheongju, Republic of Korea
Search for more papers by this authorSium Ahmed,
Abdullah Mohammad Shohael,
Kee Yoeup Paek,
Sium Ahmed
Cell Genetics and Plant Biotechnology Laboratory, Department of Biotechnology and Genetic Engineering, Jahangirnagar University, Savar, Dhaka, 1342 Bangladesh
Search for more papers by this authorCorresponding Author
Abdullah Mohammad Shohael
Cell Genetics and Plant Biotechnology Laboratory, Department of Biotechnology and Genetic Engineering, Jahangirnagar University, Savar, Dhaka, 1342 Bangladesh
Correspondence
Abdullah Mohammad Shohael, Department of Biotechnology and Genetic Engineering, Jahangirnagar University, Savar, Dhaka-1342, Bangladesh.
Email: [email protected]
Search for more papers by this authorKee Yoeup Paek
Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University, Cheongju, Republic of Korea
Search for more papers by this authorFirst published: 08 October 2021
Abstract
The purpose of the present study was to evaluate the growth potential and some rarely reported bioactivities (antioxidant, thrombolytic, anticoagulant, and anthelmintic) of Panax ginseng C.A. Meyer adventitious roots. To demonstrate the growth, shake flask and laboratory-scale bioreactor cultures have been employed. The obtained biomass was dried and extracted with water, ethanol, and methanol. The growth ratio (12.62 ± 1.03) observed in the bioreactor was significantly higher than in the shake flask culture. The presence of 10 different phytochemical classes, including carbohydrates, saponins, glycosides, and terpenoids were detected in qualitative estimation. Significant quantities of phenolics, flavonoids, proteins, and tannins were determined. Dose-dependent antioxidant activities were observed, and the IC50 values of methanolic and ethanolic extracts were very similar to the standard. The highest (29.26 ± 5.31%) thrombolytic potential was shown by the methanolic extract. The ethanolic extract significantly extended the coagulation times up to 2.5 fold. The highest anthelmintic properties in terms of paralyzing (2.21 ± 0.31 min) and killing (3.69 ± 0.41 min) of the parasitic worms were displayed by the aqueous extract. The in vitro root growth implies the commercial feasibility of ginseng production in Bangladesh and the demonstration of potential bioactivities strengthens medicinal implications and also offering new research areas.
CONFLICT OF INTEREST
The authors report no conflict of interest.
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