Volume 42, Issue 4 p. 418-430
Research Communication

Curcuma longa polyphenols improve insulin-mediated lipid accumulation and attenuate proinflammatory response of 3T3-L1 adipose cells during oxidative stress through regulation of key adipokines and antioxidant enzymes

Axelle Septembre-Malaterre

Axelle Septembre-Malaterre

Inserm, UMR 1188 Diabète athérothrombose Thérapies Réunion Océan Indien, Plateforme CYROI 2 rue Maxime, 97490 Sainte-Clotilde, La Réunion, France

Université de La Réunion, UFR Santé, 1 Allée des Aigues Marines, 97487 Saint-Denis, La Réunion, France

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Fanny Le Sage

Fanny Le Sage

Inserm, UMR 1188 Diabète athérothrombose Thérapies Réunion Océan Indien, Plateforme CYROI 2 rue Maxime, 97490 Sainte-Clotilde, La Réunion, France

Université de La Réunion, UFR Santé, 1 Allée des Aigues Marines, 97487 Saint-Denis, La Réunion, France

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Sarah Hatia

Sarah Hatia

Inserm, UMR 1188 Diabète athérothrombose Thérapies Réunion Océan Indien, Plateforme CYROI 2 rue Maxime, 97490 Sainte-Clotilde, La Réunion, France

Université de La Réunion, UFR Santé, 1 Allée des Aigues Marines, 97487 Saint-Denis, La Réunion, France

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Aurélie Catan

Aurélie Catan

Inserm, UMR 1188 Diabète athérothrombose Thérapies Réunion Océan Indien, Plateforme CYROI 2 rue Maxime, 97490 Sainte-Clotilde, La Réunion, France

Université de La Réunion, UFR Santé, 1 Allée des Aigues Marines, 97487 Saint-Denis, La Réunion, France

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Laurent Janci

Laurent Janci

Coopérative Agricole des Huiles Essentielles de Bourbon, 83 rue Kervéguen, 97430 Le Tampon, La Réunion, France

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Marie-Paule Gonthier

Corresponding Author

Marie-Paule Gonthier

Inserm, UMR 1188 Diabète athérothrombose Thérapies Réunion Océan Indien, Plateforme CYROI 2 rue Maxime, 97490 Sainte-Clotilde, La Réunion, France

Université de La Réunion, UFR Santé, 1 Allée des Aigues Marines, 97487 Saint-Denis, La Réunion, France

Address for correspondence: Prof. Marie-Paule Gonthier, PhD, Inserm, UMR 1188 Diabète athérothrombose Thérapies Réunion Océan Indien, Plateforme CYROI 2 rue Maxime, 97490 Sainte-Clotilde, La Réunion, France. Tel: 262 693 92 08 55; Fax: 262 262 93 88 01; E-mail: [email protected].Search for more papers by this author
First published: 20 April 2016
Citations: 24

Abstract

Plant polyphenols may exert beneficial action against obesity-related oxidative stress and inflammation which promote insulin resistance. This study evaluated the effect of polyphenols extracted from French Curcuma longa on 3T3-L1 adipose cells exposed to H2O2-mediated oxidative stress. We found that Curcuma longa extract exhibited high amounts of curcuminoids identified as curcumin, demethoxycurcumin, and bisdemethoxycurcumin, which exerted free radical-scavenging activities. Curcuma longa polyphenols improved insulin-mediated lipid accumulation and upregulated peroxisome proliferator-activated receptor-gamma gene expression and adiponectin secretion which decreased in H2O2-treated cells. Curcuminoids attenuated H2O2-enhanced production of pro-inflammatory molecules such as interleukin-6, tumor necrosis factor-alpha, monocyte chemoattractant protein-1, and nuclear factor κappa B. Moreover, they reduced intracellular levels of reactive oxygen species elevated by H2O2 and modulated the expression of genes encoding superoxide dismutase and catalase antioxidant enzymes. Collectively, these findings highlight that Curcuma longa polyphenols protect adipose cells against oxidative stress and may improve obesity-related metabolic disorders. © 2016 BioFactors, 42(4):418–430, 2016