Ketone bodies mimic the life span extending properties of caloric restriction

The extension of life span by caloric restriction has been studied across species from yeast and Caenorhabditis elegans to primates. No generally accepted theory has been proposed to explain these observations. Here, we propose that the life span extension produced by caloric restriction can be duplicated by the metabolic changes induced by ketosis. From nematodes to mice, extension of life span results from decreased signaling through the insulin/insulin‐like growth factor receptor signaling (IIS) pathway. Decreased IIS diminishes phosphatidylinositol (3,4,5) triphosphate (PIP3) production, leading to reduced PI3K and AKT kinase activity and decreased forkhead box O transcription factor (FOXO) phosphorylation, allowing FOXO proteins to remain in the nucleus. In the nucleus, FOXO proteins increase the transcription of genes encoding antioxidant enzymes, including superoxide dismutase 2, catalase, glutathione peroxidase, and hundreds of other genes. An effective method for combating free radical damage occurs through the metabolism of ketone bodies, ketosis being the characteristic physiological change brought about by caloric restriction from fruit flies to primates. A dietary ketone ester also decreases circulating glucose and insulin leading to decreased IIS. The ketone body, d‐β‐hydroxybutyrate (d‐βHB), is a natural inhibitor of class I and IIa histone deacetylases that repress transcription of the FOXO3a gene. Therefore, ketosis results in transcription of the enzymes of the antioxidant pathways. In addition, the metabolism of ketone bodies results in a more negative redox potential of the NADP antioxidant system, which is a terminal destructor of oxygen free radicals. Addition of d‐βHB to cultures of C. elegans extends life span. We hypothesize that increasing the levels of ketone bodies will also extend the life span of humans and that calorie restriction extends life span at least in part through increasing the levels of ketone bodies. An exogenous ketone ester provides a new tool for mimicking the effects of caloric restriction that can be used in future research. The ability to power mitochondria in aged individuals that have limited ability to oxidize glucose metabolites due to pyruvate dehydrogenase inhibition suggests new lines of research for preventative measures and treatments for aging and aging‐related disorders. © 2017 The Authors IUBMB Life published by Wiley Periodicals, Inc. on behalf of International Union of Biochemistry and Molecular Biology, 69(5):305–314, 2017

decreased IIS. The ketone body, D-b-hydroxybutyrate (D-bHB), is a natural inhibitor of class I and IIa histone deacetylases that repress transcription of the FOXO3a gene. Therefore, ketosis results in transcription of the enzymes of the antioxidant pathways. In addition, the metabolism of ketone bodies results in a more negative redox potential of the NADP antioxidant system, which is a terminal destructor of oxygen free radicals. Addition of D-bHB to cultures of C. elegans extends life span. We hypothesize that increasing the levels of ketone bodies will also extend the life span of humans and that calorie restriction extends life span at least in part through increasing the levels of ketone bodies. An exogenous ketone ester provides a new tool for mimicking the effects of caloric restriction that can be used in future research. The ability to power mitochondria in aged individuals that have limited ability to oxidize glucose metabolites due to pyruvate dehydrogenase inhibition suggests new lines of research for preventative measures and treatments for aging and aging-related disorders. V C 2017 The Authors IUBMB Life published by Wiley Periodicals, Inc. on behalf of International Union of Biochemistry and Molecular Biology, 69(5): [305][306][307][308][309][310][311][312][313][314]2017 This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. Abbreviations: D-bHB, D-b-hydroxybutyrate; HIF-1a, hypoxia-inducible factor 1-alpha; IGF, insulin/insulin-like growth factor; FOXO, forkhead box transcription factor; HDAC, histone deacetylase; IIS, insulin/insulin-like growth factor receptor signaling pathway; ILP, insulin like protein; IST-1, insulin receptor substrate-1; Nrf2, Nuclear factor (erythroid-derived 2)-like 2; PGC1a, peroxisome proliferator-activated receptor gamma coactivator 1-alpha; PDH, pyruvate dehydrogenase; PDK1,4, pyruvate dehydrogenase kinase isozyme 1 or 4; PDPK1, phosphoinositide-dependent kinase-1; PI3K, phosphoinositide 3 kinase; PIP2, phosphatidylinositol (4,5)-bisphosphate; PIP3, phosphatidylinositol (3,4,5) triphosphate; PTEN, phosphatase and tensin homolog; RNS, reactive nitrogen species; ROS, reactive oxygen species; SASP, senescence-associated secretory phenotype; SIRT1, sirtuin 1; SOD, superoxide dismutase Keywords: ketone bodies; aging; lifespan; Caenorhabditis elegans; NADPH; FOXO3a; reactive oxygen species

Ketone Bodies and Extension of Life Span
In 1935, McCay et al. showed that caloric restriction of 30% to 50% increased the average life span of rats from 500 to 820 days (1). Since that time, caloric or dietary restriction has been shown to increase life span in a wide variety of species, from yeast (2) to nematodes (3) to fruit flies (4) to mice (5) and primates. In studies of primates, calorie restriction was shown to extend lifespan by one group (6), but an earlier study using a slightly different calorie restriction protocol did not find an effect on lifespan (7). A number of proposed mechanisms for the phenomena have been suggested including: retardation of growth, decreased fat content, reduced inflammation, reduced oxidative damage, body temperature, and insulin signaling, and increase in physical activity and autophagy (8). However, no coherent mechanistic explanation has been generally accepted for this widely observed phenomenon that caloric restriction extends life span across the species. Yet, an obvious metabolic change associated with caloric restriction is ketosis. Increased ketone body concentrations occur during caloric restriction in widely different species ranging from Caenorhabditis elegans (9) to Drosophila (4) to man where ketone bodies are produced in liver from free fatty acids released from adipose tissue (10).
Ketone bodies were first found in the urine of subjects with diabetes (11) creating in physicians the thought that their presence was pathological. However, Cahill showed that ketone bodies were the normal result from fasting in man (12), where they could be used in man in most extrahepatic tissue including brain (13). The ketone bodies, D-b-hydroxybutyrate (D-bHB) and its redox partner acetoacetate are increased during fasting (14), exercise (15), or by a low carbohydrate diet (16). Originally ketone bodies were thought to be produced by a reversal of the b-oxidation pathway of fatty acids. However, it was definitively and elegantly shown by Lehninger and Greville that the b-hydroxybutyrate of the b oxidation pathway was of the L form while that produced during ketogenesis was the D form (17). This fundamental difference in the metabolism of the D and L form of ketone bodies has profound metabolic effects.
The metabolism of the D-form results in oxidation of the mitochondrial co-enzyme Q couple (18) and an increase in the redox span between the mitochondrial NAD and Q couples with a resultant increase in the DG 0 of adenosine triphosphate (ATP). The L-form of b-hydroxybutyrate is activated by conversion of ATP to adenosine monophosphate (AMP), a more energetically costly process than the activation by succinyl-CoA. In contrast to the metabolism of D-bHB, which produces only NADH, the further metabolism of the L-form is metabolized by the fatty acid b-oxidation system, which results in the reduction of one mitochondrial NAD and one co-enzyme Q with no increase in redox span between the two couples and therefore no increase in the DG 0 of ATP hydrolysis. When catabolized for the synthesis of ATP in mitochondria, D-bHB produces more ATP per oxygen molecule consumed than many other respiratory substrates due to this unique nature of D-bHB metabolism (18,19).
Blood ketone bodies can also be elevated, without elevation of blood free fatty acids by ingestion of ketone body esters such as D-bHB-R 1,3 butanediol monoester (20). This ketone ester has been evaluated for toxicity (21) and is recognized as generally recognized as safe (GRAS) by the Food and Drug Administration.
Recently, it was shown that administration of D-bHB to C. elegans caused an extension of life span resulting in that ketone body to be presciently labeled as "an anti-aging ketone body" (9). In the same experiment, L-b-hydroxybutyrate failed to extend life span. If it is accepted that the ketone body, D-bHB is an "anti-aging" compound, this could account for the widespread observation that caloric restriction, and its resultant ketosis, leads to life span extension. Many of the signaling pathways mediating extension of life span have been determined by geneticists largely by work using the short-lived nematode C. elegans.

Genetic Mechanisms of Life Span Extension
There are marked heritable differences in median life span between species: from less than 3 weeks in C. elegans, between 2 and 3 years for the mouse or rat, 10 to 15 years for the dog, around 70 years for humans, and up to over 400 years in a bivalve mollusk Artica islandica. This observation makes it clear that life span has a heritable component.
The first genetically induced increase in life span in C. elegans was reported for a mutation in age-1, encoding a catalytic subunit of the of phosphatidylinositol 3-OH kinase of the insulin/insulin-like growth factor (IGF) receptor signaling (IIS) pathway (22) (23). The Kenyon laboratory insightfully took experimental advantage of the short life span of C. elegans to identify mutations in the abnormal dauer formation-2 (daf-2) insulin/IGF-1 receptor gene of the IIS pathway that led to a twofold increase in C. elegans life span (24). This life span extension was found to be predominately caused by the decreased phosphorylation and nuclear translocation of the DAF-16/FOXO transcriptional regulator leading to expression of over 200 genes including those involved in metabolism, proteostasis, and antioxidant defenses (25,26) (Fig. 1). Activation of other factors such as the SKN-1/Nrf2 transcriptional regulator also contributes to the longevity effects of reduced IIS under certain experimental conditions (27). The increased life span of the daf-2 mutant could be further increased by dietary restriction indicating at least partially distinct mechanisms of action (28).
It was at first puzzling why a mutation or decreased expression of the daf-2 insulin/IGF-1 receptor gene in C. elegans was associated with extension of life span. After all, IGF causes proliferation in cells that are not postmitotic and hypertrophy in myocytes. It is ironic, given the attraction to sweet taste, that consumption of glucose with resultant stimulation of the insulin receptor should be a signal for shortening life span. Decreased signaling through this pathway likely evolved as a mechanism to delay reproduction until food was more abundant and the delay in reproduction carried with it an increase in survival. More understandable is the finding that an increase in the activity of the DAF-16/FOXO protein, controlling expression of several antioxidant enzymes and heat shock proteins should play a key role in life span extension. However, as animals have evolved longer life spans, FOXO proteins have evolved additional more complex roles in regulating cellular function and aging including stimulating apoptosis (29) that likely helps prevent tumorigenicity (30). FOXO proteins are modified post-translationally by acetylation and phosphorylation, which are regulated by many factors including metabolism, inflammation, and oxidative stress (29). The complex roles and signaling mechanisms thought to control the expression and activity of various FOXO proteins have recently been reviewed (31). An allele of FOXO3a, one of four human FOXO genes, is associated with extreme longevity in humans (32). Longevity may be regulated through other specific signaling pathways and transcriptional regulators such as mechanistic target of rapamycin (mTOR), 5 0 AMP-activated protein kinase (AMPK), sirtuin 1 (SIRT1), sirtuin 3 (SIRT3), nuclear factor (erythroid-derived 2)-like 2 (Nrf2), and peroxisome proliferator-activated receptor gamma coactivator 1-a (PGC-1a) (33), although nematode orthologs of PGC-1a and SIRT3 appear to be absent.
The longevity effect from mutation of genes in the IIS pathway was discovered in C. elegans. This longevity effect was also later found in fruit flies and mice. In C. elegans, the normal signaling pathway results in phosphorylation and sequestration in the cytosol of the forkhead box transcription factor DAF-16. IGF-1 receptor, IGF-1R, is a tyrosine kinase receptor. Insulin-like peptides or IGFs are ligands for IGF-1R (DAF-2 in C. elegans and dInR in Drosophila) stimulating its autophosphorylation and recruitment of adaptor proteins (insulin receptor substrate-1 (IST-1), AGE-1, CHICO, Shc (Src homology and collagen protein), Insulin receptor substrate-1 (IRS-1), and Insulin receptor substrate-2 (IRS-2)), which activate a class I phosphoinositide 3-kinase. This leads to an increased concentration of PIP3 in the plasm membrane. PTEN and the orthologue dPTEN and DAF-18 are antagonists reducing the concentration of PIP3. High membrane concentrations of PIP3 stimulate the signaling cascade by inducing PDPK1 phosphorylation of AKT-1, which in turn phosphorylates the various FOXO transcription factors. Abbreviations: AGE-1 is a phosphoinositide 3-kinase (PI3K), PIP3 stands for phosphatidylinositol (3,4,5)-triphosphate, PIP2 is the abbreviation for phosphatidylinositol (4,5)-bisphosphate, PTEN is phosphatase and TENsin homolog, PDPK1 is phosphoinositide-dependent kinase-1, AKT is named for the Ak mouse strain, and the t stands for thymoma. Dauer is German meaning endurance or duration, indicating a suspended developmental stage. DAF is the family of proteins that were investigated as dauer factors that regulate entrance into the dauer state, CHICO means small boy in Spanish referring to the small body size of the mutant flies. mTORC2 is a complex containing mechanistic target of rapamycin (mTOR). It is made up of mTOR, Rictor, mLST8, Protor1/2, Sin1, and Deptor. FOXO indicates a forkhead box transcription factor. ILP is the abbreviation for insulin-like peptides. IGF-1 stands for insulin-like growth factor-1. In the 1950s, Harman postulated that the toxicity of reactive oxygen species (ROS) was central to the mechanism of aging (34) as it was to radiation toxicity (35). There has been accumulating evidence since then that the mechanism limiting the life span results from ROS damage (36,37). Later, data (37) greatly support the mitochondrial free radical theory of aging. The first is the strong inverse correlation between mitochondrial ROS production and longevity, and the second is the strong inverse correlation between the degree of fatty acid unsaturation in tissue membranes and longevity among related species. No other parameters measured corresponded with life span as well as these indicators, which likely evolved to minimize ROS-mediated damage.
While ROS and reactive nitrogen species (RNS) are necessary for certain signaling pathways, their unregulated production is destructive leading to pathology. However, in the last 10 years evidence from C. elegans and other model organisms The NADPH system of antioxidant enzymes and NADPH-dependent molecular antioxidants. The two primary pathways providing sufficient electron donors for the reduction of oxidized species in the cytosol, organelles, and membranes are shown. This is accomplished, in part, through NADPH-dependent reduction of glutathione (GSH), vitamin C (Vit C), and vitamin E (Vit E). The redox potential of these secondary systems are all set by the redox potential of the free cytosolic [NADP 1 ]/[NADPH] system to which they are linked enzymatically.

FIG 2
has demonstrated that ROS-mediated signaling is required for some mechanisms of life span extension.
The toxicity of ROS/RNS is ameliorated by the NADPH system (Fig. 2), the redox potential of which is made more negative by the metabolism of ketone bodies (19,38,39). The redox potential of the free cytosolic [NADP 1]/ [NADPH] system is about 20.42 V, about the same redox potential as hydrogen and is the most negative redox potential in the body (38). Other reducing agents, such as the ascorbic acid couple, are linked enzymatically to the [NADP 1 ]/[NADPH] couple (19). In peripheral tissues in the fed state, NADPH is primarily produced from glucose metabolism by the hexose monophosphate pathway (40). During fasting when glucose is limiting, NADPH is produced from the metabolism of ketone bodies in the Krebs cycle (18,39) mainly through the action of NADP-dependent isocitrate dehydrogenase. During calorie restriction, mitochondrial SIRT3 deacetylates and activates the NADP-dependent isocitrate dehydrogenase IDH2 leading to increased NADPH production and an increased ratio of reduced-to-oxidized glutathione in mitochondria (41). Both FOXO1 and FOXO3a induce expression of IDH1 (42), a cytoplasmic form of NADP-dependent isocitrate dehydrogenase. Citrate or isocitrate formed from ketone body catabolism in mitochondria can be exported by the citrate-isocitrate carrier to the cytoplasm for the production of NADPH by IDH1 (Fig. 2).
Addition of reducing agents such as ascorbic acid did not uniformly increase the life span of model organisms, perhaps because these treatments had both pro and antioxidant effects or that reducing agents block ROS signals required for life span extension. Extravagant claims for the beneficial effects of high doses of ascorbic acid made by Linus Pauling were largely unsubstantiated. It was through the work of Krebs and Veech that the control of redox states in the cell and the dominant role of the free [NADP 1 ]/[NADPH] was appreciated (38). The detoxification of free radicals is dependent upon the multiple redox couples which are linked to and whose redox potential is set by the most negative NADP system (19). In the absence of malnutrition, there is little or no reason to take antioxidant supplements because the ability of these compounds to function as antioxidants is largely determined by the [NADP 1 ]/[NADPH]. As aluded to above, this ratio is regulated by the flux of substrates through enzymes that generate or consume NADP 1 and NADPH, the reduction of which is brought about by the metabolism of ketone bodies. Therefore, consuming increased amounts of antioxidants has little effect on the [NADP 1 ]/[NADPH].
Data in apparent conflict with the free radical theory of aging has led to its increased scrutiny. In C. elegans, one study showed that increasing free radicals by knocking out the superoxide dismutase (SOD) genes one at a time did not shorten life. Knockout of sod-4, an extracellular protein, even had the counter-intuitive effect of extending life span (43). Another group showed that mice engineered to overexpress SOD and catalase did not live longer than normal (44). However, overexpressing mitochondrial-targeted catalase in mice did lead to life span extension (45). SOD and catalase are both dismutases that are not linked to the NADPH system of clearing ROS/RNS that we hypothesize to be the most important driving force for life span extension.
There is substantial data supporting the ability of decreased [NADP 1 ]/[NADPH] to extend life span. Glucose-6phosphate dehydrogenase overexpression increased median life span in Drosophila (46) and female mice (47). Longer-lived strains of Drosophila were shown to possess higher glucose-6phosphate dehydrogenase activity than shorter lived strains (48). By increasing flux through NADP-dependent forms of the enzyme, knocking out or knocking down NAD-dependent isocitrate dehydrogenase increased life span in yeast (49) and C. elegans (50). Finally, overexpression of NADP-dependent malic enzyme extended life span in Drosophila (51). Studies should now be performed to more directly test the hypothesis that increased NADPH levels extend lifespan through bolstering the NADPH antioxidant system.

Decreased Pyruvate Dehydrogenase Activity in Aged Tissues is Bypassed by Ketone Body Metabolism
Aging has been shown to lead to decreased mitochondrial pyruvate dehydrogenase (PDH) complex activity. In heart, this decreased activity was not due to lower PDH complex levels, but due to increased phosphorylation that inhibits complex activity (52). PDH phosphatases, which are able to increase PDH activity, have been shown to be stimulated by insulin (53), and in skeletal muscle, this stimulation was shown to decline with age, but be restored by exercise (54). Decreased PDH activity has been found in specific regions of aged brain such as in the striatum and brain stem as a result of increased PDH kinase activity (55). This finding could result from increased ROS production from mitochondria during aging that increases hypoxia-inducible factor 1-alpha (HIF-1a) activity (56) and the expression of the pyruvate dehydrogenase kinase isoform 4 (PDK4) (57). Consistent with it playing a role in the aging process, the PDH complex has also been shown to regulate cellular senescence (58). However, too much PDH activity may have pro-aging effects through a hyperstimulation of mitochondrial metabolism resulting in increased ROS production. Therefore, FOXO proteins have evolved to induce expression of PDH kinases such as PDK4 to negatively regulate PDH activity and ROS production (59). During fasting and caloric restriction, the FOXO-mediated expression of PDK enzymes may also serve an important role in the shunting of pyruvate and/or lactate to cell types or tissues with the highest energy needs, such as neurons that cannot oxidize fatty acids.
Studies in C. elegans also support a role for PDH activity in the regulation of longevity. Inhibiting PDH kinase activity with dichloroacetate extended life span (60), while overexpression of the PDH phosphatase, PDP-1, also increased longevity through increased DAF-16/FOXO nuclear translocation (61). It is important to determine whether PDH activity regulates the activity of FOXO proteins in humans. This could potentially occur through nuclear localized PDH providing acetyl-CoA for histone acetylation (62) of FOXO promoters. If PDH activity stimulates FOXO expression, declining PDH activity during aging may lead to a downstream loss of FOXOmediated transcriptional events and increased oxidative stress. As metabolism of ketone bodies bypasses PDH activity as shown in Fig. 3, ketone or ketone ester supplementation may be able to mitigate metabolic and transcriptional alterations resulting from decreased PDH activity to promote longevity. Calorie restriction was shown to stimulate skeletal muscle mitochondrial pyruvate metabolism by increasing expression of the mitochondrial pyruvate carrier and decreasing expression of the lactate dehydrogenase A gene (63). The longevity effects of caloric restriction in mammals require the FOXO3a gene (64).

Telomere Shortening is Linked to Cellular Redox Status and Metabolism
The work of Hayflick and Moorhead (65) pointed out that shortening of the telomeres set a limit to the number of divisions cells in culture could undergo before senescence occurs. Expression of the telomerase enzyme in certain germ and progenitor cells provides a solution to replicate the ends of linear chromosomes, so that the chromosomes do not become shorter with each new round of DNA replication. Telomeres are lengthened by starvation (66) and shortened by ROS damage (59). These observations are consistent with aging being a function of reactive oxygen and its reversal a function of the increasing redox potential of the NADPH system brought about by caloric restriction. The FOXO protein FOXO1 was shown to be essential for the calorie restriction-mediated increase in telomerase subunit expression (67). As cells approach their Hayflick limit, the expression of the FOXO genes FOXO1 and FOXO4 have also been shown to decline (68), which would lead to decreased SOD2 and catalase expression. Senescent cells and tissues not only show decreased function but also acquire a senescence-associated secretory phenotype (SASP), a pro-inflammatory, pro-aging state. Mitochondrial dysfunction that increases ROS/RNS production also induces a cellular senescence program with a modified SASP (67).

Other Potential Anti-Aging Mechanisms of Ketone Bodies
Decreased insulin signaling activates mammalian FOXO proteins such as FOXO1 and FOXO3, which stimulates expression of many genes involved in autophagy (69). In addition, decreased insulin signaling or nutrient deprivation inactivates mTOR kinase to stimulate autophagy, which is required for dietary restriction-mediated life span extension in C. elegans (70). Consistent with these effects, D-bHB treatment has been shown to stimulate autophagy in cultured cortical neurons (71) and increased rates of autophagy are likely one of the several molecular mechanisms that contribute to the life span extending effects exerted by ketone bodies. One mechanism through which D-bHB may decrease IIS to activate FOXO proteins and autophagy is through a direct inhibition of AKT (72). This inhibition may also be responsible for the fasting-induced insulin resistance observed in muscle, heart, and other peripheral tissues that preserves glucose use for the brain (73,74). D-bHB may also exert protective metabolic effects by binding at least two different G protein-coupled receptors, HCAR2/ Gpr109/PUMA-G (first discovered to be a nicotinic acid receptor) and free fatty acid receptor 3 on the plasma membrane (75). As these genes evolved in chordates and are not present in invertebrates, they could not function in the evolutionarily conserved role of ketone bodies in life span extension. But activation of these receptors likely plays important metabolic signaling roles mediated by D-bHB. Finally, the gut microbiome plays an important role in providing substrates for ketone body synthesis (76) and could therefore effect the extent of ketone body synthesis during caloric restriction to influence the magnitude of life span extension, but a further discussion of this research topic is beyond the scope of this review.

Feeding Ketone Esters
One effect of feeding rats with the ketone ester, D-bHB-R 1,3 butanediol monoester, was a 1.7-fold decrease in blood glucose and over a twofold decrease in blood insulin (77). The same decreases in glucose and insulin are seen after feeding ketone esters to mice (78). These metabolic changes induced by feeding ketone esters mimic the decreased IIS induced by a longevity-inducing mutation in daf-2 in the nematode (23,26).
Ketone body metabolism bypasses decreased PDH activity in aged tissues. FIG 3 In addition to mutations in daf-2 that increase nuclear translocation and activity of DAF-16/FOXO, there are ways to increase the transcription of FOXO genes metabolically. For example, in mammals, the transcription of FOXO3a can be induced by inhibition of class I and IIa histone deacetylases (HDACs) by D-bHB (79) or possibly by b-hydroxybutyrylation (80) (Fig. 4). Inhibition of these HDACs by D-bHB induces the expression of other antioxidant and detoxification genes such as the metallothionein-1 (MTL1) that can lead to reduced ROS toxicity. In liver, fasting also increases FOXO1 activity through a mechanism where glucagon stimulates class IIa HDAC translocation to the nucleus to recruit the class I HDAC HDAC3 to deacetylate FOXO1 (81) These pathways also affect metabolism, phosphorylation potential, redox states, and the ability to clear ROS. In C. elegans, the life span extension induced by administration of the ketone body D-bHB required nematode homologs of AMPK, SIRT1, FOXO, and Nrf2. No additional increase in life span was observed for D-bHB treatment to a long-lived S6 kinase mutant of the target of rapamycin (TOR) signaling pathway suggesting that TOR inhibition also plays a role in the ketone body-mediated longevity effects (33).
As somewhat distinct from genetic manipulation of the IIS pathway, feeding ketone bodies results in the reduction of the free cytosolic [NADP 1 ]/[NADPH] ratio (39), which provides the thermodynamic force required to reduce glutathione and other antioxidant couples that destroy oxygen free radicals (19). The metabolism of ketone bodies, which lower both blood glucose and insulin, decrease the activity of the IIS pathway that in turn leads to an increase in the level and activity of the unphosphorylated FOXO transcription factors central for life span extension (26). Ketosis, which is a common consequence of caloric restriction, may provide an explanation for why caloric restriction leads to life span extension in most species. Here, we propose that the life span extension produced by caloric restriction can be duplicated by the metabolic changes induced by ketosis.

Conclusion
Aging in man is accompanied by deterioration of a number of systems. Most notable are a gradual increase in blood sugar and blood lipids, increased narrowing of blood vessels, an increase in the incidence of malignancies, the deterioration and loss of elasticity in skin, loss of muscular strength and physiological exercise performance, deterioration of memory and cognitive performance, and in males decreases in erectile function. Many aging-induced changes, such as the incidence of malignancies in mice (82), the increases in blood glucose and insulin caused by insulin resistance (39,78), and the muscular weakness have been shown to be decreased by the In the well-fed state, the FOXO3a transcription factor is prevented from entering the nucleus by phosphorylation. FOXO3A is marked for degradation by ubiquitin (Ub). DNA with FOXO3a promoter remains out of reach due to lysine (K1) interacting with negative charges in DNAs phosphodiester backbone keeping histones in the condensed state. In a state of ketosis, HDAC is inhibited by D-b-hydroxybutyrate. The acetyl (Ac-) group neutralizes the charge on lysine opening the histone complex exposing the FOXO3a promoter and upregulating superoxide dismutase (MnSOD), catalase, and metallothionein MT. An alternative mechanism proposed by Xie et al. is shown in the third panel.

FIG 4
Veech et al metabolism of ketone bodies (18,83), a normal metabolite produced from fatty acids by liver during periods of prolonged fasting or caloric restriction (12).
The unique ability of ketone bodies to supply energy to brain during periods of impairment of glucose metabolism make ketosis an effective treatment for a number of neurological conditions which are currently without effective therapies. Impairment of cognitive function has also been shown to be improved by the metabolism of ketone bodies (84). Additionally, Alzheimer's disease, the major cause of which is aging (20) can be improved clinically by the induction of mild ketosis in a mouse model of the disease (85) and in humans (86). Ketosis also improves function in Parkinson's disease (87) which is thought to be largely caused by mitochondrial free radical damage (19,88). Ketone bodies are also useful in ameliorating the symptoms of amyotrophic lateral sclerosis (89). It is also recognized that ketosis could have important therapeutic applications in a wide variety of other diseases (90) including Glut 1 deficiency, type I diabetes (91), obesity (78,92), and insulin resistance (20,39,93), and diseases of diverse etiology (90).
In addition to ameliorating a number of diseases associated with aging, the general deterioration of cellular systems independent of specific disease seems related to ROS toxicity and the inability to combat it. In contrast increases in life span occur across a number of species with a reduction in function of the IIS pathway and/or an activation of the FOXO transcription factors, inducing expression of the enzymes required for free radical detoxification ( Figs. 1 and 2). In C. elegans, these results have been accomplished using RNA interference or mutant animals. Similar changes should be able to be achieved in higher animals, including humans, by the administration of D-bHB itself or its esters.
In summary, decreased signaling through the insulin/IGF-1 receptor pathway increases life span. Decreased insulin/IGF-1 receptor activation leads to a decrease in PIP 3 , a decrease in the phosphorylation and activity of phosphoinositidedependent protein kinase (PDPK1), a decrease in the phosphorylation and activity of AKT, and a subsequent decrease in the phosphorylation of FOXO transcription factors, allowing them to continue to reside in the nucleus and to increase the transcription of the enzymes of the antioxidant pathway.
In mammals, many of these changes can be brought about by the metabolism of ketone bodies. The metabolism of ketones lowers the blood glucose and insulin thus decreasing the activity of the IIS and its attendant changes in the pathway described above. However, in addition ketone bodies act as a natural inhibitor of class I HDACs, inducing FOXO gene expression stimulating the synthesis of antioxidant and metabolic enzymes. An added important factor is that the metabolism of ketone bodies in mammals increases the reducing power of the NADP system providing the thermodynamic drive to destroy oxygen free radicals which are a major cause of the aging process.